Career Stories

Career Stories: Chapter 18

In this chapter of Career Stories, we bring you the story of  Radhika Vaishnav, who has donned various hats in her stellar career so far- as an R&D consultant, a SciComm enthusiast (teaching and mentoring), a college faculty, and an executive editor for the International Journal of Molecular and Immuno Oncology. In this candid discussion, she talks about how she pursued teaching and mentoring during her academic journey, and shares her opinions about the relevance of science communication for a researcher at any level.

What directed you to pursue science communication after professorship tenures at different institutes, mentoring young scientists, and academic editing?

I followed the traditional path during my initial career days. I did my BSc in Zoology and MSc in Biochemistry from MSU, Vadodara in the 90s. Then, I went to the US, because at that time you required 4 years of UG in order to pursue your PhD. I began my PhD at Loyola University Chicago, took a break for personal reasons (marriage and moving around different US cities) and subsequently rejoined my PhD program at the University of Kentucky at Lexington, Kentucky. The first PhD stint ended in a Masters degree on Molecular biology in cancer. It also led to my very first publication in the same field.

Despite my initial traditional track, my career meandered a bit from the usual. I settled in Lexington for my doctorate and postdoctorate. After that, I moved to Louisville, Kentucky for a full-time faculty position at the University of Louisville. Then, I decided to move back to India. Although I did not continue my track as a full-time faculty, I realized I was missing scientific interaction and communication that was integral to an academic scientist’s role. So a year or two after moving back to Vadodara, India, I took to mentoring clinicians locally and nationally in data analysis, presentation and writing of research articles for publication in  journals.   

I started actively carrying out academic editing in the capacity of a freelancer. I had always enjoyed writing and communicating. In fact, after high school, in the early 90s, one of the options I had considered for a career was journalism. I loved Biology, though, and in those days, doing the sciences alongside humanities was not even given as an option in school or college in India. My father was a strong orator, writer and was a Hemato-oncologist who opened up my mind to the possibility of Molecular Biology and its’ immense future.

Two decades later, I have merged my love of language with my depth in life sciences as a scientific writer, editor and educator.

What influenced you to choose a specialized subject like molecular biology for your second master’s degree in the US; after obtaining your UG and PG degrees from India in interdisciplinary subjects?

Once I finished my UG and PG degrees from MSU, Vadodara, India; I decided I wanted to get into molecular biology. I got influenced by 3 dynamic teachers. The first one was in my UG, Prof. Nene who taught me Biochemistry. She was a dedicated teacher who spent her weekends teaching us biochemical pathways out in the college corridors in an unconventional manner.

I was also blessed to have inspiring teachers in my PG; who were limitless in their teaching capabilities. For example, there was Prof. Acharya who had a science book club where we read many books authored by great scientists such as Feynman, Richard Dawkins, etc. We also put together a library in our Biochemistry department on the basis of student contribution of buying a book every month. Over the course of my masters, me and my fellow classmates had built a science library together; which was an inspiring activity for all.

Prof. Harish Padh had formed a small group of people from Vadodara called the “Biotechnology Interest Group.” He would invite all of us college students to come and attend their seminars. Additionally, a few of us would have a “Business in Biotechnology” session where we would indulge in student-driven round table discussions about various topics in the business of biotechnology.

I had excellent teachers and professors who would think outside the box and help me dream about what I could achieve. This, along with my own parents being in the field of life sciences, directed me to consider a career in biotechnology or molecular biology.

Molecular biology in cancer was in its baby stages with knowledge being more theoretical than practical since the protocols were still being established. That was why I chose molecular biology and I was fortunate to get into a PhD program at the Loyola University of Chicago Cancer Center in 1998. I began my work under Dr L Miele wherein I was studying the role of NOTCH-1 in transformation of normal cells into malignant ones. Around that time, telomerase, RAS, and other oncogenes/tumor suppressors were just being understood. It was an exciting time, as it paved the way for future targeted therapies that we all see today. I put my Ph.D. on hold when I married and moved for a couple years to Portland where I worked as a research scientist at the Department of Molecular Medicine, Oregon Health and Sciences University. My husband and I took turns in supporting each other in our career ambitions.

How did these foundational degrees contribute to your teaching/mentorship experience?

I think having a foundational degree in life sciences helped me a lot because I have been able to identify with students at all levels and from all walks of life. Not having a specialized curriculum from the beginning gives you a lot of versatility to formulate a career in science communication or teaching. It helps to develop a very broad understanding because you are able to move into, communicate and understand any area in the biological sciences.  On this, you can steadily build and specialize if you choose to go into research.

You never know about the future, one door opens, another closes. It helps to have a variety of different experiences even if we do choose a research path. Hence, over the years please do keep a couple of other interests going on. Don’t just put all your apples in one basket because life is uncertain. Something interesting may come up and you may want to change your direction in the future. I have mentored many students and I am firm about being flexible and having a solid knowledge base. Knowledge is important but you also need a lot more than that to go through life.

Was it providence or an informed choice that brought you to pursue molecular neuroscience in neurodegeneration/injury for your PhD and Post-doctoral research?

After Portland, we moved to Lexington, Kentucky where my husband Dr. Anand Vaishnav and I both pursued our academic track. We stayed with a friend while interviewing there, and she introduced me to a scientist couple who eventually became my PhD guides i.e. Dr. Tom & Dr. Marilyn Getchell. Initially, I joined their lab as a research analyst and was responsible for overseeing all the students and managing all the research activities in the lab. Eventually, I decided to continue with my PhD which I had halted when I left Loyola. Since their work was in molecular degeneration and regeneration of the olfactory system, I chose a project in that field and finally started my PhD at Sanders Brown Center on Aging, University of Kentucky. It was an interesting environment where they were working on Alzheimer’s disease and aging to find early markers of the former. Autopsy or biopsy samples from humans and mouse models were utilized. Eventually, I got more interested in bioenergetics of neurons because of my keen interest in mitochondria associated with oxidative damage that can occur in the nervous system due to internal or external factors, causing degeneration of these cells.

My postdoctoral research was awarded an individual NIH fellowship on the topic of “Therapeutic Strategies for Neurodegeneration and Spinal Cord Injury” under Dr Edward Hall, Spinal Cord and Brain Injury Research Center (SCOBIRC), University of Kentucky.

As per your previous interview from the Young Scientist Leader (YSL) program organized by Dr Felix Bast, you have participated in many certification programs and science outreach activities since your undergraduate years. How do you see such (perceived) extra-curricular activities being of help to aspiring researchers or PhD students?

I would not prefer to use the term ‘extracurricular activities’ here, because I believe once you are pursuing doctoral or postdoctoral research, you are no longer a traditional student. You are not attending classes only to take exams. You are actually more of an apprentice during this time. Any other skills that you wish to acquire, can also be pursued. It can be an individual or group activity. I have done numerous such activities throughout my education and career.

For example, while I was pursuing my postdoc, we (myself and Dr. Theresa Thomas) had built together a postdoctoral group called SCoBIRC Postdoc Group. This driving force for the formation of this group was a realization that there is teaching and mentoring all throughout one’s education, but by the time you are a postdoctoral fellow, sometimes you lose that structure of going to a PI and acquiring guidance. So here, in this group, we had regular meetings and we would take turns presenting, especially if someone had an upcoming job interview or any kind of conference presentation. We would be the peers/teammates/classmates who would listen to and critique each other. We made it a very collegial and constructive activity.

In the US, the doctoral and postdoctoral communities are very multicultural. We would also have occasional social activities like Potluck and multicultural programs. All these activities are not exactly ‘extracurricular’, but they enrich the already existing curriculum.

Science communication stems from being able to communicate what you do. It has to start with you being able to explain what you do. I had a professor at Loyola University, Dr. Katherine Knight who used to tell us that one should be able to explain their research in a limited time- 1 minute, 5 minutes, 10 minutes, or max. 30 minutes. Everything does not have to be a long-winding presentation or a 45-minute lecture. If someone asks you to explain your research in under 5 minutes to them, you should be able to pitch your research and grab the audience’s attention by making it interesting as well as wrapping it up within the time limit.

While I was a faculty at the University of Louisville, my department (Department of Physiology, with Joint appointment in Neurology and Neurosurgery) sponsored a certification program in entrepreneurship with Kauffman Foundation where we would meet experts and were encouraged to take an idea from its conception to visualization/startup. Here, we were told to sell our idea in either 11 mins or 1 min elevator pitches. Initially, we did the latter. We were critiqued and trained in the same manner all throughout the course.

Elevator pitches include meeting laymen/potential investors in an elevator and your response to their question of “Hey! What do you do?” Depending on what you say in the next minute, the other person will make a decision right there whether they want to listen to you or not. So, you can imagine putting together everything you want to say within this minute, is not a cakewalk. Starting up the Journal IJMIO, working with the team, expanding it and currently stewarding it into a legacy for clinician-scientists to communicate has been possible thanks to these lessons learnt.

During my PhD, I started teaching in a community college on the weekends over the topic of Physiology. I had learnt it in depth over the years because the broad subject of my PhD was the Physiology despite my research area being neuroscience. I continued over the years to teach Physiology along with other Life Science subjects at colleges including my current affiliation as Adjunct Faculty, Life Sciences, IvyTech Community College, Indianapolis, Indiana.

So these types of things are always happening in parallel. You are not just doing one thing. You are teaching, communicating, building something, trying to put together a startup company, failing at it, while you are doing doctoral or postdoctoral research or being a faculty member. Someone did ask me to join a new steering committee to try to put together a novel neuroscience course with a very new faculty. Despite all the challenges, I did that. I was the one who would hardly say no to any self-improvement opportunities within the field. This is why I would not call these activities ‘extracurricular’. Hobbies and artistic activities can be considered ‘extracurricular activities’. But activities like science communication, being able to prepare and deliver your research pitch are co-curricular, skill-building and life-changing activities, that can actually strengthen your career profile and who you are. In the future, one or all of these will make you unique as opposed to only gaining knowledge through education.

How did you get associated with IJMIO (International Journal of Molecular and Immuno Oncology)? Please introduce us to your journey there

I did not get introduced to IJMIO but rather, I was one of the founding members of the journal.

So, in 2016, I was attending a molecular oncology conference with my father, an oncologist, Dr. Divyesh Mehta, who was giving a talk and he introduced me to his friend Dr. Purvish Parikh who was there as a faculty member. I told him about how I wanted to do something different – to leave a legacy. He offered the role of honorary executive editor at the International Journal of Molecular and Immuno Oncology.  The journal was to be launched soon and he was putting together a small start-up team, which I joined.

Our goal at the journal is to encourage people who are from a clinical background, to come out of their comfort zone and actually write and communicate their work. Many people are still not communicating whatever they are observing in their clinics or their research. Hence, there is so much potential to do more. We also give encouragement to new authors and look forward to their work being published. The journal is the official journal of the Molecular Oncology Society under the current able leadership of editor Dr. Randeep Singh and a multispecialty team of oncologists on the editorial board.

It has been a very exciting activity for me because I am at the forefront of a field that is continuously undergoing a lot of change and development. So, I really chose to do this to keep myself on my toes as I am forced to read and learn constantly and stay active.

According to you, what improvements can be made in education, mentoring, and research environments in India to be on par with the USA?

Knowledge is not what is setting India apart from the US or any other country. You need to have an environment that supports or encourages fundamental research. Without the society getting interested or involved, the funding will cease.

In the US, funding comes largely from the NIH, NSF, and other similar agencies which are government-based or private foundations (Eg: Bill and Melinda Gates Foundation). These funds come from the public i.e. taxpayer’s money or donations. Basically, you need to empower the people and you can only do that by giving them knowledge and making them feel comfortable about research. Now, in India, there are people who are willing to donate money to hospitals, NGOs for the underprivileged, religious activities, education, children, food drives, etc. But having an actual system where everyone pays taxes and a certain percent of taxes go into funding research automatically will encourage research job creation as well as encourage our research talent to stay within our country. This change has to come from the grassroot level. For that, the public has to be included in the entire story of scientific research. For that, you have to communicate science to them: what you are doing and why you are doing it. The COVID-19 pandemic did bring that change in communication.

Moreover, science is connected. It has no boundaries. I do not look at science as being limited by national borders. I do not know if it is an unpopular opinion or not, but honestly when you are a pure scientist, you think only about science. It does not matter where you live or work. But scientists are people who have families– children, parents. Hence, we want to stay in a certain place. Once you do that, you need to sustain yourself. However noble you may feel your profession is, you want to do research and solve problems, you do need to be able to support your family. If you cannot, you will leave science or research, for sure. You will find something else because obviously you are smart and hardworking enough to get into a masters or a PhD.

The only thing that is very sad is that we have already spent a significant part of our youth preparing ourselves to be a scientist. When it comes to supporting your family, if you need to either leave the country or change your profession, then something is just not quite right yet in India. I think that we need more awareness and that is where science communication begins to gain importance. 

How is this scenario changing in India, with years passing by?

Internet, globalization, and access to knowledge have been groundbreaking for research in India. Internet has been a great equalizer; so people from all over the world have access to the same knowledge. You have YouTube videos, and online PDFs of textbooks today which are very different from our days. Back then, we had to go to the library and find interesting articles from the paperback journals that were subscribed to. We were sometimes limited by the availability of resources in the library. Since all we had were paper-based journals, we had to make do with whatever was available. Those days at the beginning of my education were very different compared to now where we have everything thanks to the Internet.

The pandemic in the last 2-3 years changed many things. For example, because of the pandemic, everyone in India and around the world came to know about RT-PCR. This was impossible before the pandemic.

It is now possible for an Indian individual who has not undergone advanced education to know what a virus is, what immunity is, what PCR is, and that you need to get vaccines. Most importantly, today the Indian public knows that you need research to solve everyday problems, and it can save lives during an emergency. This is what is needed. I think we did an excellent job of communication during the pandemic. This is the spirit that needs to continue. We need to build up that energy in our own country where people love science and research and want to support it.

Please enlighten students (like myself) about notable research domains/ SciComm ventures to be explored.

Science policy is a critical area where SciComm is very valuable. Many opportunities are available globally for individuals who can act as a bridge between scientists and the community/government. Another notable field is working for large foundations. Companies such as pharmaceuticals and technology-based require content creation for education, marketing and awareness. Any of these roles would require strong communication skills.

Knowing multiple languages is a great advantage in science communication as scientific writers who can reach across languages are highly valuable. I would suggest following Wellcome Alliance, India Bioscience, United Nations, WHO, EMBO, AAAS and various international foundations which are devoted to scientific communication.

Follow your heart honestly in terms of what field to pursue. Ignore what other people say. Don’t put all your eggs in one basket. Add to the experience, build on it, and make yourself unique. Don’t settle or get disheartened in the case of bad mentors or professors. That’s a learning experience too. Everything adds to your experience. Take it in a positive way, cut your losses, and move on. When you have a positive mentor or a positive experience, take it in your stride. Get as much out of it as you can. However, do not be just a taker, be a giver as well. While you are learning, give back your knowledge to society by communicating. Take a few students under your wing and pass on any knowledge/skills that you have gained so far. It is extremely rewarding and essential for humankind and the future. We share what we know.

Experiences like the pandemic have taught us life is short and uncertain. All that knowledge is of no use if we have not shared it. My plea to everyone is that regardless of whatever field you choose- be it academia, any private profession, or a biotech company, please do not stop communicating in the form of teaching and mentoring. Prepare students for tomorrow at any level. There is no work that is too small for scientists. If you can take a few 5-year-olds and make them excited about something, that is absolutely fine as you are already doing a noble deed. You are already doing enough to show others that you are capable of bringing about a positive change. Getting educated is fine, but giving back is invaluable and absolutely priceless. I fervently request you all to do it.

You have been my mentor for many projects in the last two years. I have always admired your calm, composed but tenacious attitude to solving problems. Could you share with us a memorable mentorship/teaching experience that has changed your perspective, and contributed towards your own learning process? 

When you are on the track of getting a PhD/becoming a scientist, you do need people who are very calm, caring, as well as resilient in your life. They continue to steer you back into the right direction. In my case, I wanted to be a scientist and a professor.

In this path, there will be many roadblocks and hurdles: your papers getting rejected and designed experiments failing most of the times. During your doctoral degree journey, you feel like a failure numerous times. On the other hand, when you go home, your family must be wondering: what in the world do you do? Why do you spend so many hours in a lab? Many a time, you may not be able to answer such questions to their satisfaction.

Usually, it is such an esoteric topic that you are working upon: one small molecule inside one small organelle and some organism that nobody in your family has ever heard of. Then you are trying to explain it in a way that they understand by telling them that it is related to a certain disease. You also want to make your response interesting to them, so that they know you are not wasting your life on something that they do not actually understand. They are proud and happy of your achievements. However, they do not really know what you do. Even my family members can only give a very vague description of anything that I ever did. Very few people in your family and friends will pick up your papers and read them. This is the life you are leading. You are living in a bubble and everyone around you is doing something else in their lives. There is a big gap.

You have a continuous impostor syndrome going on during your doctorate. After months of framing and validating your thesis, you are faced with your PhD defense viva panel and the audience. As an ambivert, you wish to not sound boring during your thesis defense.

Hence, one of my memorable mentorship experiences was with my PhD mentor Dr. Thomas Getchell. We worked during evenings and weekends to improve my PhD presentation through inculcating various tips provided by him. His gestures of caring and taking time to improve my presentation helped me tremendously. One of his tips were that during a presentation, in order to sound knowledgeable, it need not be long but it must be interesting. The details should be kept out of the presentation or else you will have slides which are just full of words which no one wants to listen to. In this way, I started to learn many presentation skills in my final year of PhD. So my final PhD defense was the first time in my life where I could speak confidently in front of an audience. Honestly, I give all credit to him. I wish I had learnt all of this earlier.

Another tip that was given to me was that I should sit in the front in an audience during a lecture and always ask one question. Perhaps my PhD mentor understood my introverted behavior, which no one else did before. I was used to the untrue feedback from my peers of being under confident, unprepared, and unready during my MSc presentations. Instead, I would love to prepare the subject beforehand thoroughly and could always write flawlessly. But during my speech in front of an audience, I used a lot of linker words like “um”, “uh” during pauses. This persists till the present day.

I just wanted to say that the memorable moments are when someone is humane despite being a great professor/researcher. If one is able to be honest about having an impostor syndrome despite being a professor, and lead by example as to how they overcame that, that honesty works like magic. This strategy works for me even today. I always project this viewpoint to my students. I always remind myself to show them that I do not know everything but I am a human and have been through a lot of things which helped me learn and gain wisdom. By this virtue, I have something to teach them as well.

Career Stories

Career Stories: Chapter 15

In this chapter of Career Stories, we bring you the story of Dr Mahita Jarjapu, who is a Bioinformatics Researcher & a postdoctoral fellow at La Jolla Institute for Immunology, San Diego, California, USA.

Being a researcher with profound bilateral sensorineural hearing loss, only fuels her sheer grit and determination to continue thriving and building a Career in Academia. She also vouches for empowering other researchers who have disabilities, to make a significant mark in STEM fields.

In this candid discussion, she speaks at length about her interdisciplinary research journey, sheds light on how she overcame the challenges faced due to her disability, while suggesting some simple, and practical measures that society at large can adopt in order to practice inclusivity. 

What inspired your  transition from a masters in chemistry to a PhD in biology?

I did my BSc in Biology (Botany, Zoology, Chemistry). I have always been interested in biology from my school days. During my BSc, I started developing an interest in Chemistry. So, during my MSc, my interest in the interface of biology and chemistry grew. Basically, I have always been interested in this because I wanted to understand how biology works at the molecular level. Molecular Biology involves protein molecules and their interactions. These interactions come under the domain of chemistry. Hence, this is what I wanted to do for my PhD.

We have divided our understanding of the universe into different disciplines (physics, chemistry, biology, and mathematics). In reality, all these principles come into play simultaneously. Presently, even in science, when we are trying to solve a problem or investigate a research question, we tend to look at it from only one point of view; we do not consider other factors that are outside our area. For example, you take the interactions between two cells. From the perspective of biology, you will observe the proteins involved in both the protein and cellular interactions. If observed through physics, cells are dynamic and not static. All these factors need to be considered. If you want a complete picture of the problem for solving it effectively, you need to look at it through the lenses of different disciplines.

Your current research journey has been filled with immunology, omics, computational protein biology, structural biology, and bioinformatics. What  influenced you to choose interdisciplinary research for your career?

As I explained earlier, it is important to look at the context or the bigger picture. So, I understand that it can be difficult because if you have done MSc in chemistry and you go and do a PhD in Biology, then you have to revise many concepts. I have to admit that in the beginning there will always be a steep learning curve but if you can overcome that, then it is worth it. I have benefitted from this. After my MSc in Chemistry, when I started my PhD, my knowledge of biology was very poor. So, when you do a PhD, you have to clear a qualifying exam after two years of enrolling into the PhD program. This qualifying exam is tough because the question paper is designed to apply your knowledge instead of being fact-based. For that, you need to have a very good understanding of biology concepts. Hence, for the first two years, I had to spend a lot of time catching up with my knowledge of biology. After I gained that knowledge, I could put together a lot of my chemistry knowledge gained from my MSc. Having these two subjects together helped me to look at the question from multiple perspectives. I think that is very important when you are approaching your problem. It will teach you how to design an experiment and will also tell you whether the experiment is feasible; or what would be the drawbacks of the experiment. The experiment will solve one question but it will also lead to another question. Hence, you have to keep all these factors in your mind. This is the reason why I wanted to work across disciplines.

My PhD was mainly in Bioinformatics but I did not have any working knowledge about coding. I had to teach myself to practice, which is not very difficult. You can learn it yourself through online courses. From personal experience, it is better to learn  anything involving wet lab research in real time. You need practical experience with a wet lab. These practical experiences are not possible to obtain when learning from online courses. But, coding languages can be learnt online for dry lab work. Initially, if you want a career in wet lab and then want to switch to dry lab, you need to have more practical experiences first. Later, you can teach yourself coding online.

For my first postdoctoral research at Dartmouth College in New Hampshire, USA, even though the focus of the lab was in computational biology, it was located in the Computer Science department. Most of my friends were computer science graduates. In my lab, we had people who were working towards a PhD degree in computer science and their research involved developing different algorithms that could be applied to address problems in biology. If you are a PhD in Biology, you would have to apply it in computer science. That was something new for me because I had to learn computer science. I had to write code and use it to generate data. The computational time and memory usage  taken by the algorithm needs to be kept in mind while writing a code for it.

Now, in my current postdoctoral research, I deal majorly with immunology. So, whatever I learnt in computer science, I apply and develop it to investigate questions in immunology.

How did your research interests lead you to your current Post-doctoral fellowship at Peters Lab, La Jolla Institute for Immunology?

When I was pursuing my PhD, it was on protein-protein interactions, in the context of an innate immunity pathway, which is the TLR signaling pathway. I studied how mutations in the TLR proteins affected their ability to interact with downstream signaling proteins. So, this led me to be interested in molecular recognition.

Antibody-antigen interactions are a type of protein-protein interaction which are much more diverse due to millions of antibodies produced by each individual. Each of these antibodies has a different specificity. So, I am curious to know how these specificities arise, and what mutations in the antibodies cause them to be specific to one antigen but not another. It is not feasible to study millions of antibodies experimentally. The Peters lab is a pioneer in the development of computational tools for addressing these types of questions in immunology.

My current project is wholly based on COVID, I have been studying antibodies that bind to the SARS-CoV-2 virus. I have also been studying the mutations of different variants of this virus: alpha, beta, gamma, and delta. These variants have their differences because of their mutations. I have been trying to understand how these mutations in these variants affect the ability of the antibodies to bind or interact with them. That is the question I am currently working on. Another question I am dealing with is basically understanding what makes antibodies specific to a particular antigen. Again, it all comes down to the molecular level. It is all about chemistry. Because protein-protein interactions are nothing but various amino acids interacting with each other. So, that is how I got interested to work here at Peters Lab. It is a fantastic place. My colleagues come from all over the world: Europe, South America, Africa, Australia, Asia. They all have their diverse perspectives on life and research. So, it is a very good learning experience here.

Your current PostDoc research lab has majorly focussed on immunoinformatics i.e. immunology and bioinformatics. How is bioinformatics applicable to research in immunology?

There are multiple examples of this.

One example from our institute is single-cell RNA sequencing. Blood samples taken from volunteers who have been vaccinated or have a specific disease can be used for extracting white blood cells/leukocytes. Single-cell sequencing of these cells, taken from blood extracted at different time intervals, produces millions of gene sequences. Bioinformatic algorithms help to cluster/align these sequences together and help identify differences in the transcription/expression of certain genes and proteins in these cells. Under-expression or over-expression of these genes signifies that they play a role in a given disease.

Another example is BCR (B cell receptor) sequencing. Antibodies are secreted forms of BCRs. Antigen-specific B cells can be isolated from different blood samples and sorted. Sequencing of these B cells provides the sequence of antibody that the particular B cell is expressing. Millions of such sequences from different individuals help researchers identify what sequences are important for an antibody to be effective against a specific antigen. The unique features of these sequences are understood. Different individuals produce different antibodies but sometimes there are commonalities in the sequences between two individuals. Such examples are types of convergent evolution – i.e. the sequence is favorable for the antibody which is why multiple individuals produce such antibody sequences. All these findings are due to the application of bioinformatics algorithms to immunology. In India, this has a lot of scope for such studies owing to its diverse population.

Depending on where humans live, whether in the North or South hemisphere, we have been exposed to different types of pathogens that influence our immunity and determine how we respond to them. In the case of India which is a tropical country, we see more cases of malaria or dengue. If Indians are infected with malaria, they have antibodies against the respective antigens. Sometimes, these antibodies have been found to interact with dengue proteins. This is an example of cross-reactivity which can be explored more in India.

Another example is understanding the cross-reactivity of T cells and antibodies to different pathogens. T cells are usually specific enough to bind/interact with a peptide from a particular antigen. The same T cell can recognize a peptide from another antigen if there are similarities between these two antigens. Bioinformatics can help here to understand the similarity in peptide sequences between these two antigens and its extent to understand cross-reactivity. So that is another way of applying bioinformatics to immunology.

As data generation increases, there is also a need to develop databases and repositories to store and access this immunological data. There are several databases for the immunology community – Immune Epitope Database, Coronavirus Antibody Database, and IMGT database, to name a few.

Many students consider the terms “bioinformatics” and “computational biology” to be the same. Please clear this misconception.

Computational biology is a broader field and encompasses bioinformatics as one of its subjects. Basically, computational biology is using computers to investigate any biological question. 

Bioinformatics is mostly related to data generation, handling, development of softwares, algorithms and databases to deal with biological data.

Another group that comes under computational biology is systems biology, which is the mathematical and theoretical calculation-based study of the dynamics of large biological systems. For example, understanding the fusion of protein molecules within a signaling pathway; understanding the dynamics of the various proteins involved, so on and so forth. So, for time 0, if you have, say x number of proteins, after time t, their number is estimated and their effect on the signaling pathway is explored. These are questions dealt in computational biology and not bioinformatics because there is not much scope for involvement of ‘data handing’ here. 

However, Bioinformatics has become increasingly important now because of the improvement in technology which has led to the generation of more data; especially proteomic data. Hence, to handle that data and to make sense of it, you need numerous innovative bioinformatic algorithms. I would recommend checking out The Human Cell Atlas as a great example.

Machine learning, on the other hand is a completely different concept. On a fundamental level, if you look at it, it is all about modeling the data. Basically, if you have the data, you will try to derive a mathematical equation from it. For example, if you have two groups: one control group and another group with diabetics, machine learning will try to develop a probability model to predict diabetes in a person. For that, you will have a dataset which will have the information of the sample population: age, gender, eating habits. Each of them will be labeled as “diabetic” or “non-diabetic.” Based on the machine learning algorithms, you will be able to derive an equation from this dataset to separate this data into these two categories. This model will be used on a new dataset to predict the risk of diabetes. This is a very simple example of machine learning.

You have a stellar record of numerous scientific publications and you have also held the position of scientific writer for “The STEM Times” newsletter for the past 4 years. How did you discover your passion for science communication? 

Writing is something I have been very good at since I was a child. I think you might be aware that I was trained at Balavidyalaya. For my training, I had to do a lot of writing and reading and liked it a lot. I caught the habit from a very young age of 4-5 years. Writing was one way of expressing myself, because talking for me was limited by the choice of words that I could use. But when I write, I can express myself much more beautifully. I have been interested in writing for a very long time. In fact, when I was in school, my teacher used to tell me not to become a scientist. She always suggested I become a journalist. However, I was interested in science.

I did not find it difficult. I enjoyed the process of writing. Even during my MSc, PhD students would request me for help in editing their thesis and I used to enjoy it. During my PhD also, I liked writing, making stories, and explaining concepts. During this time, I realized that there is a disconnect between the public and what we scientists do. I felt that it is important to make them understand what we are doing. It is very important to be able to explain to others in a very simple way so that they understand the importance of what we are doing. So that’s how I ended up working for “The STEM Times.” The focus of STEM Times was to basically convey scientific concepts to people in a simplified format.

During my bachelors and masters, I used to write a blog. I used to write about my experiences at NCBS through poems. People would tell me how they liked my writing and then it struck me how I could use my fondness for writing to communicate science and research.

Your previous interviews that outline what you are and how you grew up, are an inspiration to many, especially for women in STEM. Please tell us one or two pieces of specific advice you wish family members, mentors, or colleagues of children with any form of disability to adopt. 

I think parents should not underestimate their children just because they have a disability. Each of us has flaws. For some of us, it is visible while for others it is hidden. I feel that humans have the ability to somehow find solutions or alternatives to an issue, quite unknowingly, if the issue does not seem big to them. It is all in the mind. The bigger we imagine our issues to be, the harder it becomes to overcome that. This can be solved by the approach of breaking down the problem into multiple steps.

Everyone has their own shortcomings. No one is perfect. However, the society we live in does not see a person with a disability as capable of doing anything. But that is not true. This mindset is similar to people noticing a single black spot on a white paper. People should be open-minded, supportive as well as encouraging towards people with disabilities, especially disabled children. This instills self-confidence in them. Above all, please do not discourage them. I understand that well-wishers and parents of these children would have a feeling of wanting to protect them but it only hurts them in the long run.

Small actions can make a lot of difference. In my own experience, my parents never discouraged me from doing whatever I wanted to do; especially when I wanted to move to a new place for my MSc. They never asked questions or doubted my ability to live on my own, far from home. They just encouraged me to study. But other people were very surprised when my parents decided to send me to IIT Madras. Such actions make a huge impression on children because it conveys that you are confident in them and their qualities. It makes them believe in themselves instead of depending upon others for their confidence.

What measures would you suggest to increase the inclusivity of researchers who are deaf, in STEM fields in India?

You should ask what they want. Researchers who are deaf should be asked what they need as it can vary from person to person. And others need to take some minimum effort to accommodate these needs. This goes a long way in helping them. Something that might seem small to you actually makes a big difference to us. The best example is – closed captions and the service of live captioning.

For example, during my education, I did not have many facilities that could have helped me. For me, it is very important to look at a person’s face while they are speaking. If they turn away and speak, I cannot understand. So it was really difficult following classes, since we did not have any captioning service. So, I had to depend a lot on my classmates for notes. During the class, I would have to look at the notes from the classmates sitting besides me and copy them down. Basically, in college you know how they teach. The professor will speak and tell us to hear and write about it simultaneously. For me, both were not possible. Either, I can look or I can write- only one thing at a time. Hence, I would listen to the professor and then once the lecture was over, I would copy the notes from my classmates. Unfortunately, you miss out on a lot of information; you do not know 100% of what was discussed in the lecture. So, I used to go to the library to catch up on whatever I missed out on. Hence, live captions are very important.

The experience during my PhD was very different from my MSc, because instead of classes, we had research, conferences, and seminars. Seminars and conferences usually consist of questions between the audience and the speaker. For me, I would miss out on most of the questions. I did not like that. All that changed when I came to the US and started using Zoom. I saw these closed captions and then finally understood what goes on in Q&A sessions. When you are asked a question, it makes you think about how to address it. For people like me who are deaf who communicate using spoken language, we need closed captions to follow meetings and seminars.

I have attended conferences in India as well as abroad. Whenever I was in India, I was saddened to see that no one would care to ask or arrange real-time captioning for me. But when I visited conferences in Canada, they asked about my needs and made arrangements for me to have live captions. Everyone was given a device and a URL link. The link had to be opened. Then the microphone in the device would hear any speech and convey it to a third person who would immediately start typing these captions and it would be visible to a person who is deaf. This system should be implemented in Indian conferences, as in Canada I could follow everything that was being spoken and it made a huge difference to me. I could understand questions which enabled me to think more about the problem or the topic. This facility is not offered either at Indian conferences or in Indian universities. I hope this facility is provided at least in top-tier institutions in India, like IIT, NCBS, TIFR, etc. – I know that it is provided in IIMs.

Another important aspect is to increase awareness and not judge a researcher because they are deaf. I hope my experiences are able to help others like me who are also pursuing their research in India.

These days, there are softwares to help people who are deaf: Live transcribe by Google. It converts speech into text. MS Windows 11 also has an inbuilt feature for converting audio into text. This feature was designed by another person who is deaf, and who was trained in the same school as mine and is currently working at Microsoft in Seattle, USA.

Career Stories

Career Stories: Chapter 7

In this chapter of Career Stories, we bring you the journey of Dr. Anil Kumar Challa who is an Adjunct Professor in the department of biology at the University of Alabama at Birmingham. Apart from this, he is also a senior scientist at Shiv Nadar University Delhi NCR.

In this candid chat he takes us through his professional journey and talks about what keeps him motivated enough to continue pursuing a Career in Academia. 

What motivated you to take science?

So, there are two answers here. One is as fundamental as being human-I think there’s this urge to find out (more about) things. 

I guess it might sound philosophical, but I think every kid is a scientist. Every infant is so curious about the world and if it is not inhibited then I believe that everybody has the potential to become a scientist in some way or the other.

But for me in terms of a career path in science sometime around sixth grade I gravitated more towards medicine/biology. Those days, it was a social thing- you either became an engineer or a doctor. And by eighth grade, I subscribed to MBBS tutorials, but did not read a single page of it. As a kid, I wasn’t studious, nor did I read textbooks or complete my homework properly. But then, I definitely enjoyed it in middle school, when we had to study different flowers. As a kid, I grew up with a backyard full of flowering plants and fruit trees. I really enjoyed my time with the plants. It was not something very special, or something that was leading me to ‘science’ per say, but I started observing and studying a lot of things by myself. Once, in a class activity, I actually took a razor blade to cut a flower  in a  longitudinal section, drew it, and  coloured it. The reason I’m mentioning this particular incident is because the activity is extremely vivid in my memory even now- so, I started enjoying the process of going through details of things. 

And then, over the years, I got really fascinated by human anatomy and forensic sciences. In the ninth grade, one of my classmates introduced me to science reporter, and I just loved those articles- the way they were written, and I was really fascinated about ballistics and all kinds of chemistry. By the time I was in 11th and 12th grade, I gave a lot of pre-medical entrances, but eventually I realized that medicine is not for me. However, I was already bitten by a general fascination towards everything ‘science’.

Once I got into my undergrad, it was very clear in my head that this is a path I wanted to follow. My learning then was not limited to life sciences as I learnt a lot about engineering and coding through two of my very good friends from the hostel. The Banaras Hindu University campus also really helped me- that it was a very generous institution in a sense that they did not restrict an undergrad’s participation in any seminar or lectures. And because of this, I got immense clarity in my second year BSc that this is the direction that I want to pursue my career in.

You pursued a BSc in agriculture and then shifted your career to molecular biology and genetics., What inspired this transition, and was it difficult?

While in my first year of under graduation, MSc biotechnology was newly introduced, and students had a craze for it. They introduced various subjects in that course. For example, biochemistry was a separate subject/paper. My seniors, who were good friends, were preparing for the MSc Biotechnology entrance exam conducted by JNU. They were reading Principles of Biochemistry by Lehninger, which seemed interesting while I conversed with them about genetics and biochemistry. Also, one of my professors who had gone to the UK to attend the Human Genome Conference returned with lots of literature which he gave my seniors. That got me fascinated, and a couple more of such incidents caught my interest in biochemistry and molecular biology. I realized that despite being an agriculture graduate I can still pursue my research career in molecular biology. This realization dawned on me when I noticed that the authors of those publications were from diverse fields and departments. So while applying for PhD programmes in the US, I kept my options open for various programs. Particularly how I got into molecular genetics at Ohio State University for PhD might seem very silly. Those were pre internet days. We had to write to the university to procure the application form, which was difficult considering we did not know their addresses. Coincidentally when I visited my friend in IIT Madras he offered me an extra form from the Ohio State University, enquiring if I was interested to apply. I had not planned to apply there but checked out their various departments, found molecular genetics very interesting and applied for my PhD, though it was not a serious attempt. And if not for Ohio university, the story would have remained the same because I was not even sure about my research problem. It was simply my fascination for molecular biology. 

I had executed a summer internship at AIIMS in the department of biophysics for two and a half months. Summer internships in those days were not common, though today, quite unfortunately, everyone has to do it. Dr. TP Singh was very encouraging. He did not question me as to why an undergrad should work in a lab, rather he permitted me to join his lab. That environment added immense value to my learnings/understanding about research. Discussions with PhD students were extremely insightful and since I was accommodated in a research scholar’s room I could learn a lot from their conversations.

So that pretty much sums up how I landed at the Department of molecular genetics. Though it was by chance, this was bound to happen since I knew I would pursue my career in this field. I just wanted to understand how life works -the molecular basis of life. 

Which books piqued your interest in science?

I was actually scared of reading books. I am saying this out loud for such kids who are like me. Until 10th I read perhaps only one book outside my syllabus. My physics teacher in Class 12 was a very interesting character, a voracious reader. We were blessed to have a teacher like him, though at that time I disliked him as a teacher as he was very old school and strict. Also, at that time, I wanted my teacher to tell me how to crack exams, and nothing else. However, would say that there is no IIT physics or IIT chemistry, there is only physics and chemistry. He would carry a book to class which he never shared with any of us.

Nevertheless, he created an intense curiosity in us to know what the book was all about. One day he came to class and said “If you are a biology student and you haven’t read this, you are not a biology student “. He was talking about In Search of the Double Helix by John Gribbin. I bought it from an old second hand book shop and that book was a page turner! I was hooked on it. Another book that made me veer towards life sciences was The Double Helix by James Watson. It was quite an expensive book but my parents bought it for me, no questions asked. I recommend these gems to all undergrads. No doubt they are controversial, involving plenty of gossip, but rest assured- you simply won’t be able to put them down, once you start reading them.

Did you ever feel like giving up on academia, and if yes then what motivated you to continue?

In my undergrad I read Lehninger’s Biochemistry cover by cover and while doing that in the reference section there were all these scientific American articles that made me realize that PhD or research in general is not a straightforward path. Culturally it has been inculcated in my mind that every journey has its challenges, but that does not mean that one should not proceed with it. Now, I remember only good things about my PhD but there are definitely one or two times that I thought “That’s it, I want to quit!” . But since I was in the US and not India, quitting meant that if it would have taken me 2-3 weeks to pack everything up and come back- and within that buffer period, something interesting always happened in the lab which made me realize that it was worth continuing and that’s how I  completed my PhD. 

There are three types of truths- “, known known”, “known unknown” and “unknown unknown”. The reason for the frustration is “unknown unknown”, with which even your guide cannot help you, and that is frustrating but you have to deal with it on your own. Understanding that- if an experiment does not work, then it’s not always the protocol that goes wrong, sometimes it’s our own mistakes too and,changing even a small component can make a huge difference- as a student we are always impatient and believe that everything should work the way it has been prescribed to us,but that is ’ not always the case. There are times when things don’t work out, and one needs to figure out why. 

After PhD, I thought about quitting research a couple of times. After my post-doctoral tenure, I could not continue with research-  but by then, I was also pursuing a career in science education and that kept me going on. Teaching is what I love doing and is now the reason I continue to remain in science.

What changes do you think are required in the current scenario of the Indian education system?

In any graduate program, not much thought is given to mentoring students. Everybody focuses on what study material should be shared. The focus is on the content and the assessment. The goal should not be in turning all students into scientists. Rather, they should be allowed to choose,while we can definitely contribute to making the journey interesting and engaging. Students should not believe it is wrong to change one’s career path or field of education. In case you are a biotechnology graduate and you see no reason nor interest in hanging onto that path, you should change your domain, confidently. It is your growth and you have to choose what is best for you. Only you  can mold yourself into the best version of you. 

The system should be more student oriented. This confidence should be instilled in students if this course is not working out for you something else will, because we all are trying for the sustenance of our society when seen as a larger picture.